This is ordered chronologically by the production order, which is why it differs a little from the order the episodes were originally broadcast.

0:00 Homer Simpson, Bart Simpson
0:24 Marge Simpson, Lisa Simpson
0:40 Maggie Simpson
1:14 Abe Simpson (Grampa)
1:44 Itchy, Scratchy
2:06 Krusty the Clown
2:40 Marvin Monroe
3:14 Moe Szyslak, Barney Gumble, Larry, Sam
3:36 Barney Gumble (speaking)
3:50 Seymour Skinner, Milhouse Van Houten, Martin Prince, Lewis Clark, Richard
4:47 Edna Krabappel
5:25 The School Psychologist
6:06 Radioactive Man
6:22 Otto Mann
6:49 Sherri, Terri, Wendell
7:00 Wendell (speaking)
7:20 Sherri (speaking), Terri (speaking)
7:43 Waylon Smithers
7:58 Clancy Wiggum, Jasper
8:22 Montgomery Burns
9:01 Eddie, Lou
9:32 Nelson Muntz
10:18 Old Jewish Man
10:29 Herman Hermann
11:13 Snowball II
11:30 Dewey Largo, Janey Powell
12:06 Janey Powell (speaking)
12:21 Bleeding Gums Murphy
12:37 Patty Bouvier, Selma Bouvier, Timothy Lovejoy
12:49 Timothy Lovejoy (speaking)
13:03 Jimbo, Kearney, Dolph
13:53 Apu Nahasapeemapetilon
14:16 Ralph Wiggum
14:35 Ned Flanders
14:57 Todd Flanders
15:25 Patty Bouvier (speaking), Selma Bouvier (speaking)
15:59 Santa's Little Helper
16:38 Rod Flanders
16:50 Lenny Leonard, Carl Carlson
17:32 Lenny Leonard (speaking)
17:41 Helen Lovejoy
18:23 Sideshow Bob
18:48 Sideshow Bob (speaking) (in disguise)
19:03 Kent Brockman
19:25 Roy Snyder (Judge)
19:41 Sideshow Bob (speaking) (not in disguise)
20:09 Agnes Skinner
20:56 Joe Quimby (Mayor)
21:09 Kang, Kodos
21:39 Dr. Julius Hibbert
22:52 Jacqueline Bouvier
23:15 Maude Flanders
23:41 Roger Meyers, Jr.
24:15 Sideshow Mel
24:29 Lionel Hutz
25:48 Blue-Haired Lawyer
23:53 Nick Riviera
27:12 Blue-Haired Lawyer (speaking)
27:26 Akira
27:57 Rainier Wolfcastle / McBain
28:34 Harlan Dondelinger
28:50 Artie Ziff
29:07 Clancy Bouvier
29:18 Raphael (Sarcastic Guy)
29:35 Troy McClure
30:13 Drederick Tatum
30:42 Bernice Hibbert
30:51 Hans Moleman
31:32 Groundskeeper Willie
32:12 Herbert Powell (baby), Mona Simpson
32:42 Herbert Powell (adult)
33:53 Professor Frink
34:21 Old Jewish Man (speaking)
34:34 Squeaky-Voiced Teen
35:06 Elizabeth Hoover
35:13 Snake Jailbird
35:29 Comic Book Guy
35:33 Comic Book Guy (speaking)
36:18 Alice Glick
36:58 Just Stamp the Ticket Man
37:17 Fat Tony, Louie, Legs
39:01 Luann Van Houten
39:38 Hyman Krustofsky (Rabbi)
40:34 Lunchlady Doris
40:43 Sideshow Mel (speak...singing)
41:00 Sanjay Nahasapeemapetilon
41:10 Arnie Pye
41:24 Sanjay Nahasapeemapetilon (speaking)
41:34 Kirk Van Houten
41:50 Bumblebee Man
42:12 Horatio McCallister (Sea Captain)
42:46 Ruth Powers
43:15 Sarah Wiggum
43:22 Gary Chalmers
43:56 Üter Zörker
44:02 Üter Zörker (speaking)
44:23 Rich Texan
44:45 Cletus Spuckler
44:59 Leopold
45:27 Luigi Risotto
45:53 Gerald Samson (The Unibrow Baby)
46:10 Mrs. Carlson
46:15 Mrs. Carlson (speaking)
46:22 Ham, Database, Cosine, Email, Report Card
47:08 Disco Stu
47:18 Brandine Spuckler
47:33 Manjula Nahasapeemapetilon (child)
47:43 Bernice Hibbert (speaking), Sarah Wiggum (speaking)
47:57 Lindsey Naegle
49:00 Duffman
50:11 Manjula Nahasapeemapetilon (adult)
50:27 Gil Gunderson, Cookie Kwan
50:57 Crazy Cat Lady
51:43 The Yes Guy
52:00 Mrs. Muntz (voice only)
52:04 Julio Franco
52:47 Mrs. Muntz (in person)
53:02 Shauna