This video talks about the Luciferase reporter assay. We will learn what is luciferase assay used for and finally, we will look at the applications of the Luciferase assay.

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0:00 Luciferase reporter assay
1:31 Promoter is cloned upstream of luciferase sequence
3:56 How to understand whether the promoter is strong or weak?
6:44 A normalized ratio is obtained for each well by calculating
7:21 The fold change in activity between two test groups using the following equation