WEIRD Fluorescence Two-Photon absorption!
Les' Lab Les' Lab
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 Published On Jun 2, 2023

Episode 54

#optics
#laser
#nonlinearoptics

Lets take a look at Two-photon absorption

In this episode, let's look at a really unusual Fluorescence phenomenon, two photon absorption!
This phenomenon is used in two photon microscopy for imaging cells buried deep in living tissue, and could be used in volumetric 3D displays and ultra dense data storage!

This was originally predicted by Maria Goeppert-Mayer in 1931 in her doctoral dissertation. Thirty years later, the invention of the laser permitted the first experimental verification of TPA.
Maria went on to become the second woman to win a Nobel Prize in physics for proposing the nuclear shell model of the atomic nucleus.

The unit for the two-photon absorption cross section is named the Goeppert Mayer (GM) unit after her.

1064 laser module: http://bit.ly/3ZTLKeZ

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0:00 Intro
0:18 Fluorescence
1:06 Maria Goeppert Mayer Two-photon absorption
1:42 Two-photon absorption explanation, Jablonski diagrams
2:43 Suitable Fluorescent Dyes
2:48 Rhodamine 6G
3:18 Single photon fluorescence
4:45 15kW IR Laser!
5.45 Extreme power density
6:00 Two Photon fluorescence!
6:58 Sneak peak and credits!

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